ABSTRACT
Objective To establish a sensitive, rapid and simple high-performance liquid chromatography (HPLC) method for the determination of amphotericin B (AraB) and itraconazole (ITZ) in human cerebrospinal fluid (CSF). Methods Solid-phase extraction (SPE) was involved in sample preparation in this method, followed by reverse-phase separation under two different chromatographic systems. Results The tinearity for both AraB and ITZ ranged from 5 ng/mL to 100 ng/mL and the inter-day and intra-day RSD were both below 7.6% at the concentrations of 20,50 and 100 ng/mL. Conclusions The method we established has been applied to the therapeutic drug monitoring on cryptococcal-meningitis-infected patients who were given comedication of these two drugs.
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AIM To study the pharmacokinetics and bioavailability of clinafloxacin in rats. METHODS The drug concentration was determined by HPLC. The main pharmacokinetic parameters were obtained by 3P87 program. An RP-C18 was used as the stationary phase. The mobile phase was a mixture of acetonitrile-0.05 mol*L-1 citric acid triethylamine (pH 2.5) (20∶80). The flow rate was 1.0 mL*min-1. The UV absorbance detector was set at 300 nm. RESULTS A good linearity was obtained from 0.03-20 μg*mL-1 of clinafloxacin in rat plasma with γ=0.9998. The plasma concentration-time curve of clinafloxacin conformed to one compartment open model. After ig administration of 50 mg*kg-1 and 100 mg*kg-1 dose of clinafloxacin in six rats, mean Cmax and AUC values increased in proportion to dose. Mean T1/2 appeared to be independent of dose. Mean AUC was 65±6 and 27±4 μg*h*mL-1 respectively after iv and ig adminostration of 100 mg*kg-1 dose. The extent of bioavailability (F) of clinafloxacin was 42%. CONCLUSION The results of the pharmacokinetic study of clinafloxacin showed that it exhibited first order kinetic characteristics and the bioavailability is low.
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Object To determine the contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in the stems and fruits of Schisandra rudriflora (Franch ) Rehd. et Wils , which were collected from different areas Methods An HPLC method was set up: Column, Sphereclone, ODS (250 mm?4 6 mm, 5 ?m); mobil phase, A: H 2O, B: MeOH; gradient elution was with 70% B from 0-4 min, 70%-100% B from 4-54 min; the flow rate was 0 4 mL/min; the column temperature was 25 ℃ and the DAD detector was used at 254 nm Results The contents of schisantherin A, deoxyschizandrin, schisandrin B and schisandrin C in S. rubriflora were 0.026%-0.083%, 0.007%-0.945%, 0.002%-0.121%, 0.010%-0.038%, respectively. Deoxyschizandrin exists widely in S. rubriflora and its content is higher in fruits than that in stems Conclusion Deoxyschizandrin is the main active lignan in the fruits of S rubriflora